{"uniprot":"A0JP26","gene":"POTEB3","name":"POTE ankyrin domain family member B3","organism":"Homo sapiens (Human)","status":"dark","length":581,"sequence":"MVAEVCSMPAASAVKKPFDLRSKMGKWCHHRFPCCRGSGKSNMGTSGDHDDSFMKTLRSKMGKCCHHCFPCCRGSGTSNVGTSGDHDNSFMKTLRSKMGKWCCHCFPCCRGSGKSNVGTWGDYDDSAFMEPRYHVRREDLDKLHRAAWWGKVPRKDLIVMLRDTDMNKRDKQKRTALHLASANGNSEVVQLLLDRRCQLNVLDNKKRTALIKAVQCQEDECVLMLLEHGADGNIQDEYGNTALHYAIYNEDKLMAKALLLYGADIESKNKCGLTPLLLGVHEQKQQVVKFLIKKKANLNALDRYGRTALILAVCCGSASIVNLLLEQNVDVSSQDLSGQTAREYAVSSHHHVICELLSDYKEKQMLKISSENSNPEQDLKLTSEEESQRLKVSENSQPEKMSQEPEINKDCDREVEEEIKKHGSNPVGLPENLTNGASAGNGDDGLIPQRKSRKPENQQFPDTENEEYHSDEQNDTQKQLSEEQNTGISQDEILTNKQKQIEVAEKEMNSKLSLSHKKEEDLLRENSMLREEIAMLRLELDETKHQNQLRENKILEEIESVKEKLLKAIQLNEEALTKTSI","function_summary":"A human scaffold protein built from tandem ankyrin repeats and a C-terminal coiled-coil that organizes multiprotein assemblies to maintain tissue integrity. By using an ankyrin solenoid core for high-affinity binding and a coiled-coil module for oligomerization and membrane-proximal tethering, it assembles adhesion and signaling complexes that support retinal tissue stability. Its association with membrane-trafficking machinery likely routes it into extracellular vesicles, positioning it to contribute to vesicle-mediated communication that sustains retinal homeostasis.","plain_language":"POTEB3 has barely been studied. The model's best guess: A human scaffold protein built from tandem ankyrin repeats and a C-terminal coiled-coil that organizes multiprotein assemblies to maintain tissue integrity. By using an ankyrin solenoid core for high-affinity binding and a coiled-coil module for oligomerization and membrane-proximal tethering, it assembles adhesion and signaling complexes that support retinal tissue stability.","uniprot_summary":"May play a role in tissue integrity.","go_terms":{"molecular_function":[{"id":"GO:0005515","label":"protein binding"}],"biological_process":[{"id":"GO:0001895","label":"retina homeostasis"}],"cellular_component":[{"id":"GO:0070062","label":"extracellular exosome"}]},"interpro":[{"id":"IPR036770","name":"Ankyrin repeat-containing domain superfamily","type":"homologous_superfamily","start":124,"end":386},{"id":"IPR039497","name":"CCDC144C-like, coiled-coil domain","type":"domain","start":470,"end":579},{"id":"IPR002110","name":"Ankyrin repeat","type":"repeat","start":143,"end":237},{"id":"IPR002110","name":"Ankyrin repeat","type":"repeat","start":238,"end":336},{"id":"IPR002110","name":"Ankyrin repeat","type":"repeat","start":337,"end":366}],"reasoning":"I begin with the domain architecture. The central portion of the protein is dominated by IPR036770 (Ankyrin repeat-containing domain superfamily, residues 124–386), within which three discrete ankyrin repeats are annotated: IPR002110 Ankyrin repeat (residues 143–237), IPR002110 Ankyrin repeat (residues 238–336), and IPR002110 Ankyrin repeat (residues 337–366). Ankyrin repeats form elongated solenoid scaffolds that create extensive protein–protein interaction surfaces; their tandem arrangement here creates a high-affinity, multivalent binding platform. This architecture causally establishes a primary molecular function of protein binding (GO:0005515), as ankyrin arrays specialize in assembling and stabilizing multiprotein complexes rather than catalyzing reactions. At the C-terminus, IPR039497 (CCDC144C-like, coiled-coil domain, residues 470–579) adds a second interaction module. Coiled-coils typically drive oligomerization and cytoskeletal or membrane-associated tethering, thereby extending the ankyrin scaffold into higher-order assemblies and positioning the complex near membranes or vesicular pathways.\n\nFrom this scaffolded core, I infer the biological role. Ankyrin-repeat scaffolds frequently tune signaling and adhesion pathways that maintain tissue integrity. The presence of a terminal coiled-coil suggests membrane-proximal assembly and trafficking competence, features that are particularly relevant in excitable tissues. The explicit linkage to tissue integrity and homeostasis in the eye is consistent with a role in retina homeostasis (GO:0001895). A mechanistic bridge exists: by organizing adhesion/signaling complexes and engaging vesicle/membrane pathways via its coiled-coil, the protein can stabilize photoreceptor and retinal pigment epithelium architectures and buffer stress pathways that preserve retinal tissue balance.\n\nCellular placement follows from the same logic. The absence of catalytic motifs and the presence of ankyrin/coiled-coil modules argue for a soluble scaffold that associates with membranes and vesicles rather than embedding in them. Such scaffolds are frequently recovered in extracellular vesicles; thus, packaging into extracellular exosomes (GO:0070062) is expected. The coiled-coil’s propensity for oligomerization and membrane tethering provides a physical basis for exosome association and secretion, aligning with a role in intercellular communication that supports tissue maintenance.\n\nIntegrating these features yields a mechanistic hypothesis. The ankyrin array nucleates a multiprotein hub that binds cytoskeletal and adhesion regulators; the coiled-coil domain oligomerizes this hub and couples it to membrane trafficking routes. In retinal cells, this assembly likely stabilizes junctional complexes and coordinates cargo sorting into intraluminal vesicles that become exosomes. Through this pathway, the protein supports retina homeostasis by both reinforcing intracellular architecture and participating in vesicle-mediated signaling.\n\nLikely interaction partners follow from the scaffold’s design and locale: cytoskeletal adaptors (e.g., spectrin/ankyrin systems), membrane–cytoskeleton linkers, and exosome biogenesis factors (e.g., ESCRT-associated proteins and tetraspanin-rich membrane microdomains). These associations would allow the ankyrin/coiled-coil platform to couple adhesion and signaling to vesicle production and secretion in retinal tissue.","category":"dark","control_evaluation":null,"validation_plan":null,"structure":{"source":"AlphaFold DB","pdb":"/structures/AF-A0JP26.pdb","alphafold":"https://alphafold.ebi.ac.uk/entry/A0JP26"},"links":{"uniprot":"https://www.uniprot.org/uniprotkb/A0JP26/entry","atlas":"/p/A0JP26"},"provenance":{"model":"BioReason-Pro (ESM3 esm3-sm-open-v1)","source_list":"Pharos Tdark","note":"AI prediction, not experimentally verified"}}