{"uniprot":"Q6GPH6","gene":"ITPRIPL1","name":"Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1","organism":"Homo sapiens (Human)","status":"dark","length":555,"sequence":"MNVDAEASMAVISLLFLAVMYVVHHPLMVSDRMDLDTLARSRQLEKRMSEEMRLLEMEFEERKRAAEQRQKAENFWTGDTSSDQLVLGKKDMGWPFQADGQEGPLGWMLGNLWNTGLFCLFLVFELLRQNMQHEPAFDSSSEEEEEEVRVVPVTSYNWLTDFPSQEALDSFYKHYVQNAIRDLPCTCEFVESFVDDLIEACRVLSRQEAHPQLEDCLGIGAAFEKWGTLHETQKFDILVPIVPPQGTMFVLEMRDPALGRRCGCVLVESECVCKREKLLGDVLCLVHHHRDPSAVLGKCSSSIKAALCTGFHLDVCKTVQWFRNMMGNAWALVAHKYDFKLSLPPSTTSCKLRLDYRSGRFLSIHLVLGVQREDTLVYLVSQAPDQEQLTSVDWPESFVACEHLFLKLVGRFAPENTCHLKCLQIILSLRQHQSLPHGASRPILTSYHFKTALMHLLLRLPLTDWAHNMLSQRLQDILWFLGRGLQQRSLHHFLIGNNFLPLTIPIPKTFRNAEPVNLFQHLVLNPKAHSQAVEEFQNLLTQVKTLPHAPLAAAP","function_summary":"A human adaptor that assembles membrane-proximal signaling complexes controlling calcium-dependent pathways. Its modular scaffold binds and organizes components of the inositol trisphosphate receptor pathway and death-receptor signaling hubs, thereby tuning endoplasmic reticulum–proximal calcium release and dampening extrinsic apoptosis. By stabilizing higher-order assemblies at membrane interfaces, it modulates broader signal-transduction circuits and restrains cell-death cascades.","plain_language":"ITPRIPL1 has barely been studied. The model's best guess: A human adaptor that assembles membrane-proximal signaling complexes controlling calcium-dependent pathways. Its modular scaffold binds and organizes components of the inositol trisphosphate receptor pathway and death-receptor signaling hubs, thereby tuning endoplasmic reticulum–proximal calcium release and dampening extrinsic apoptosis.","uniprot_summary":"May be involved in signal transduction and apoptosis.","go_terms":{"molecular_function":[{"id":"GO:0005515","label":"protein binding"}],"biological_process":[{"id":"GO:0007165","label":"signal transduction"},{"id":"GO:0008625","label":"extrinsic apoptotic signaling pathway via death domain receptors"},{"id":"GO:1902042","label":"negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"}],"cellular_component":[{"id":"GO:0016020","label":"membrane"}]},"interpro":[{"id":"IPR046906","name":"Mab-21-like, HhH/H2TH-like domain","type":"domain","start":418,"end":495},{"id":"IPR024810","name":"MAB21L/Cyclic GMP-AMP synthase-like receptor","type":"family","start":221,"end":541},{"id":"IPR026250","name":"Inositol 1,4,5-trisphosphate receptor-interacting protein-like","type":"family","start":313,"end":327},{"id":"IPR026250","name":"Inositol 1,4,5-trisphosphate receptor-interacting protein-like","type":"family","start":394,"end":407},{"id":"IPR026250","name":"Inositol 1,4,5-trisphosphate receptor-interacting protein-like","type":"family","start":413,"end":429},{"id":"IPR026250","name":"Inositol 1,4,5-trisphosphate receptor-interacting protein-like","type":"family","start":443,"end":458},{"id":"IPR026250","name":"Inositol 1,4,5-trisphosphate receptor-interacting protein-like","type":"family","start":473,"end":489},{"id":"IPR026250","name":"Inositol 1,4,5-trisphosphate receptor-interacting protein-like","type":"family","start":505,"end":519}],"reasoning":"I begin with the InterPro architecture. A central-to-C-terminal block is defined by IPR024810 (MAB21L/Cyclic GMP-AMP synthase-like receptor family, residues 221–541), which establishes a cGAS/cGLR-like scaffold that typically forms nucleotide-handling or signaling folds used for macromolecular assembly rather than classical catalysis. Embedded within this region is IPR046906 (Mab-21-like, HhH/H2TH-like domain, residues 418–495). HhH/H2TH-like modules are compact helix-hairpin-helix elements that stabilize nucleic-acid or polyanion-rich interfaces and often mediate high-affinity protein–protein contacts in signaling assemblies. Upstream and overlapping with this core, a series of IPR026250 family signatures (Inositol 1,4,5-trisphosphate receptor-interacting protein-like, residues 313–327; 394–407; 413–429; 443–489; 473–489; 505–519) collectively annotate a conserved IP3R-interacting protein-like platform. The repeated IP3RIP-like hits across residues 313–519 indicate a modular, likely disordered interaction hub that binds and regulates the IP3 receptor pathway.\n\nThis ordered layout—an IP3R-interacting platform (IPR026250) wrapped by a cGAS/cGLR-like scaffold (IPR024810) and reinforced by an HhH/H2TH-like binding core (IPR046906)—causes the protein to function primarily as a multivalent adaptor. Such an adaptor architecture dictates molecular function as protein binding (GO:0005515): the HhH/H2TH-like domain provides a high-affinity binding surface, while the cGAS-like fold and IP3RIP-like segments create specificity toward endoplasmic reticulum Ca2+-signaling assemblies.\n\nFrom this binding-centric chemistry, the biological role follows. The IP3R-centered interaction network governs ER-to-cytosol Ca2+ flux, which in turn modulates cell survival pathways. By assembling and tuning IP3R-dependent Ca2+ microdomains, the protein can dampen death-receptor signaling and suppress caspase activation, aligning with negative regulation of extrinsic apoptotic signaling via death-domain receptors (GO:1902042). At the same time, its modulation of Ca2+ release pathways situates it within the extrinsic apoptotic signaling pathway via death-domain receptors (GO:0008625), where it acts as a brake rather than an amplifier. The cGAS/cGLR-like scaffold further supports a role in signal transduction (GO:0007165), integrating Ca2+ cues with downstream effectors.\n\nCellular placement is inferred from the need to interface with ER-resident IP3 receptors and membrane-proximal signaling. The absence of transmembrane motifs and the presence of interaction-rich domains indicate a soluble factor that associates with membranes through partner binding. This supports a membrane-associated localization (GO:0016020 membrane), most plausibly at ER–plasma membrane contact sites where IP3R-driven Ca2+ release couples to death-receptor platforms.\n\nMechanistically, I hypothesize that the protein assembles a membrane-proximal complex that bridges IP3R with death-domain receptor adaptors and apoptotic regulators. The IP3RIP-like region recruits IP3 receptor isoforms and positions them near receptor platforms; the HhH/H2TH-like core stabilizes higher-order assemblies; and the cGAS/cGLR-like scaffold confers the geometry needed to sequester or reroute Ca2+ signals. Likely partners include IP3 receptor isoforms and death-receptor pathway components (e.g., FADD and caspase-8 adaptors), as well as ER Ca2+ handling machinery. Through these assemblies, the protein suppresses extrinsic apoptosis while broadly tuning Ca2+-dependent signal transduction.","category":"dark","control_evaluation":null,"validation_plan":null,"structure":{"source":"AlphaFold DB","pdb":"/structures/AF-Q6GPH6.pdb","alphafold":"https://alphafold.ebi.ac.uk/entry/Q6GPH6"},"links":{"uniprot":"https://www.uniprot.org/uniprotkb/Q6GPH6/entry","atlas":"/p/Q6GPH6"},"provenance":{"model":"BioReason-Pro (ESM3 esm3-sm-open-v1)","source_list":"Pharos Tdark","note":"AI prediction, not experimentally verified"}}