{"uniprot":"Q6H9L7","gene":"ISM2","name":"Isthmin-2","organism":"Homo sapiens (Human)","status":"dark","length":571,"sequence":"MRALRDRAGLLLCVLLLAALLEAALGLPVKKPRLRGPRPGSLTRLAEVSASPDPRPLKEEEEAPLLPRTHLQAEPHQHGCWTVTEPAAMTPGNATPPRTPEVTPLRLELQKLPGLANTTLSTPNPDTQASASPDPRPLREEEEARLLPRTHLQAELHQHGCWTVTEPAALTPGNATPPRTQEVTPLLLELQKLPELVHATLSTPNPDNQVTIKVVEDPQAEVSIDLLAEPSNPPPQDTLSWLPALWSFLWGDYKGEEKDRAPGEKGEEKEEDEDYPSEDIEGEDQEDKEEDEEEQALWFNGTTDNWDQGWLAPGDWVFKDSVSYDYEPQKEWSPWSPCSGNCSTGKQQRTRPCGYGCTATETRTCDLPSCPGTEDKDTLGLPSEEWKLLARNATDMHDQDVDSCEKWLNCKSDFLIKYLSQMLRDLPSCPCAYPLEAMDSPVSLQDEHQGRSFRWRDASGPRERLDIYQPTARFCLRSMLSGESSTLAAQHCCYDEDSRLLTRGKGAGMPNLISTDFSPKLHFKFDTTPWILCKGDWSRLHAVLPPNNGRACTDNPLEEEYLAQLQEAKEY","function_summary":"A secretory-pathway glycoprotein that assembles an extracellular adhesion and signaling scaffold to restrain angiogenic drive and bias cells toward differentiation and apoptosis. It folds and matures in the endoplasmic reticulum lumen before deployment, where a thrombospondin repeat and an AMOP module cooperate to bind extracellular partners with high avidity. This binding-centric mechanism suppresses cell population growth, dampens fibroblast proliferation, promotes apoptotic signaling, and supports epidermal development by tuning receptor and matrix interactions.","plain_language":"ISM2 has barely been studied. The model's best guess: A secretory-pathway glycoprotein that assembles an extracellular adhesion and signaling scaffold to restrain angiogenic drive and bias cells toward differentiation and apoptosis. It folds and matures in the endoplasmic reticulum lumen before deployment, where a thrombospondin repeat and an AMOP module cooperate to bind extracellular partners with high avidity.","uniprot_summary":"Inhibits angiogenesis.","go_terms":{"molecular_function":[{"id":"GO:0005515","label":"protein binding"}],"biological_process":[{"id":"GO:0008285","label":"negative regulation of cell population proliferation"},{"id":"GO:0045684","label":"positive regulation of epidermis development"},{"id":"GO:2001235","label":"positive regulation of apoptotic signaling pathway"},{"id":"GO:0048147","label":"negative regulation of fibroblast proliferation"}],"cellular_component":[{"id":"GO:0005788","label":"endoplasmic reticulum lumen"}]},"interpro":[{"id":"IPR036383","name":"Thrombospondin type-1 repeat superfamily","type":"homologous_superfamily","start":321,"end":376},{"id":"IPR005533","name":"AMOP domain","type":"domain","start":396,"end":559},{"id":"IPR051867","name":"Angiogenesis Inhibitor and Adhesion GPCR","type":"family","start":127,"end":571},{"id":"IPR000884","name":"Thrombospondin type-1 (TSP1) repeat","type":"repeat","start":329,"end":371}],"reasoning":"I begin with the domain architecture. The central region contains IPR036383 (Thrombospondin type-1 repeat superfamily, residues 321–376) and a nested IPR000884 (Thrombospondin type-1 [TSP1] repeat, residues 329–371). TSP1 repeats are compact β-rich adhesive modules that bind extracellular matrix components and cell-surface receptors, classically mediating anti-angiogenic and pro-adhesive interactions. Immediately C-terminal to the TSP1 module lies IPR005533 (AMOP domain, residues 396–559), an extracellular adhesion/signaling domain frequently found in secreted or membrane-associated proteins that organize cell–cell and cell–matrix contacts. The broader family signature IPR051867 (Angiogenesis Inhibitor and Adhesion GPCR family, residues 127–571) spans most of the mature ectodomain, tying together the TSP1 and AMOP modules into an extracellular adhesion/inhibitory scaffold. The ordered layout—TSP1 repeat followed by AMOP within a family that specializes in angiogenesis inhibition—creates a high-avidity extracellular binding platform rather than a catalytic core.\n\nThis architecture dictates molecular function as multivalent protein binding (GO:0005515). The TSP1 repeat provides high-affinity docking to extracellular ligands and receptors, while the AMOP domain stabilizes adhesive assemblies and transduces signals. Together, these domains cause avidity-driven complex formation that modulates downstream pathways without intrinsic enzymatic activity.\n\nFrom this binding-centric mechanism, the biological processes follow. TSP1 modules are archetypal anti-angiogenic elements; by constraining endothelial signaling and matrix engagement, they suppress proliferation and promote apoptosis when angiogenic cues are excessive. This causal role aligns with negative regulation of cell population proliferation (GO:0008285) and positive regulation of apoptotic signaling pathway (GO:2001235). The AMOP-containing ectodomain also supports tissue remodeling and stratification cues, consistent with positive regulation of epidermis development (GO:0045684). Its adhesive signaling can dampen stromal growth, matching negative regulation of fibroblast proliferation (GO:0048147). Collectively, the TSP1–AMOP tandem orchestrates anti-angiogenic and pro-differentiation programs by tuning extracellular receptor networks.\n\nCellular location is inferred from the secretory architecture and the presence of ER-lumenal maturation. The annotated lumenal residence (GO:0005788 endoplasmic reticulum lumen) indicates that the polypeptide enters the secretory pathway, folds and assembles its extracellular modules in the ER before trafficking onward. The absence of intracellular catalytic motifs and the dominance of extracellular adhesion domains argue that the mature functional pool operates outside the cell or at the cell surface after secretion.\n\nMechanistically, I hypothesize that the protein assembles an anti-angiogenic adhesive complex. The TSP1 repeat nucleates binding to matrix and cell-surface receptors, while the AMOP domain stabilizes higher-order assemblies that attenuate pro-angiogenic signaling and bias cells toward differentiation and apoptosis. Likely partners include integrins and heparan sulfate proteoglycans that mediate adhesion, as well as endothelial and epithelial receptors whose signaling is tuned by this scaffold. In the ER, transient chaperone interactions would facilitate folding of the TSP1–AMOP ectodomain prior to secretion and surface display.","category":"dark","control_evaluation":null,"validation_plan":null,"structure":{"source":"AlphaFold DB","pdb":"/structures/AF-Q6H9L7.pdb","alphafold":"https://alphafold.ebi.ac.uk/entry/Q6H9L7"},"links":{"uniprot":"https://www.uniprot.org/uniprotkb/Q6H9L7/entry","atlas":"/p/Q6H9L7"},"provenance":{"model":"BioReason-Pro (ESM3 esm3-sm-open-v1)","source_list":"Pharos Tdark","note":"AI prediction, not experimentally verified"}}