{"uniprot":"Q6NUJ1","gene":"PSAPL1","name":"Proactivator polypeptide-like 1","organism":"Homo sapiens (Human)","status":"dark","length":521,"sequence":"MLCALLLLPSLLGATRASPTSGPQECAKGSTVWCQDLQTAARCGAVGYCQGAVWNKPTAKSLPCDVCQDIAAAAGNGLNPDATESDILALVMKTCEWLPSQESSAGCKWMVDAHSSAILSMLRGAPDSAPAQVCTALSLCEPLQRHLATLRPLSKEDTFEAVAPFMANGPLTFHPRQAPEGALCQDCVRQVSRLQEAVRSNLTLADLNIQEQCESLGPGLAVLCKNYLFQFFVPADQALRLLPPQELCRKGGFCEELGAPARLTQVVAMDGVPSLELGLPRKQSEMQMKAGVTCEVCMNVVQKLDHWLMSNSSELMITHALERVCSVMPASITKECIILVDTYSPSLVQLVAKITPEKVCKFIRLCGNRRRARAVHDAYAIVPSPEWDAENQGSFCNGCKRLLTVSSHNLESKSTKRDILVAFKGGCSILPLPYMIQCKHFVTQYEPVLIESLKDMMDPVAVCKKVGACHGPRTPLLGTDQCALGPSFWCRSQEAAKLCNAVQHCQKHVWKEMHLHAGEHA","function_summary":"A human soluble, multi-repeat saposin-like protein that binds membranes and lipoprotein assemblies to remodel vesicular and extracellular membranes. By organizing lipid-rich surfaces and assembling transient protein complexes, it supports vesicle and lysosome-related trafficking and exerts broad cytoprotective effects. Its anti-apoptotic activity arises from stabilizing intracellular membranes and sequestering pro-apoptotic assemblies, consistent with a pool in cytoplasmic vesicles and lysosome-related compartments and a secreted fraction that associates with extracellular vesicles and lipoproteins.","plain_language":"PSAPL1 has barely been studied. The model's best guess: A human soluble, multi-repeat saposin-like protein that binds membranes and lipoprotein assemblies to remodel vesicular and extracellular membranes. By organizing lipid-rich surfaces and assembling transient protein complexes, it supports vesicle and lysosome-related trafficking and exerts broad cytoprotective effects.","uniprot_summary":"Has a cytoprotective activity.","go_terms":{"molecular_function":[{"id":"GO:0005515","label":"protein binding"}],"biological_process":[{"id":"GO:0043154","label":"negative regulation of cysteine-type endopeptidase activity involved in apoptotic process"}],"cellular_component":[{"id":"GO:0005829","label":"cytosol"}]},"interpro":[{"id":"IPR011001","name":"Saposin-like","type":"homologous_superfamily","start":61,"end":143},{"id":"IPR011001","name":"Saposin-like","type":"homologous_superfamily","start":182,"end":254},{"id":"IPR011001","name":"Saposin-like","type":"homologous_superfamily","start":291,"end":368},{"id":"IPR011001","name":"Saposin-like","type":"homologous_superfamily","start":393,"end":471},{"id":"IPR003119","name":"Saposin A-type domain","type":"domain","start":19,"end":59},{"id":"IPR008139","name":"Saposin B type domain","type":"domain","start":60,"end":144},{"id":"IPR007856","name":"Saposin-like type B, region 1","type":"domain","start":63,"end":99},{"id":"IPR008139","name":"Saposin B type domain","type":"domain","start":180,"end":258},{"id":"IPR008139","name":"Saposin B type domain","type":"domain","start":290,"end":370},{"id":"IPR007856","name":"Saposin-like type B, region 1","type":"domain","start":292,"end":329},{"id":"IPR008138","name":"Saposin B type, region 2","type":"domain","start":334,"end":366},{"id":"IPR008139","name":"Saposin B type domain","type":"domain","start":392,"end":473},{"id":"IPR008138","name":"Saposin B type, region 2","type":"domain","start":437,"end":469},{"id":"IPR003119","name":"Saposin A-type domain","type":"domain","start":475,"end":515},{"id":"IPR021165","name":"Saposin, chordata","type":"family","start":2,"end":511},{"id":"IPR008373","name":"Saposin","type":"family","start":19,"end":43},{"id":"IPR008373","name":"Saposin","type":"family","start":48,"end":68},{"id":"IPR008373","name":"Saposin","type":"family","start":78,"end":95},{"id":"IPR008373","name":"Saposin","type":"family","start":130,"end":148},{"id":"IPR051428","name":"Pulmonary surfactant-associated protein B-like","type":"family","start":181,"end":471},{"id":"IPR008373","name":"Saposin","type":"family","start":294,"end":314},{"id":"IPR008373","name":"Saposin","type":"family","start":342,"end":360},{"id":"IPR008373","name":"Saposin","type":"family","start":393,"end":415},{"id":"IPR008373","name":"Saposin","type":"family","start":425,"end":447},{"id":"IPR008373","name":"Saposin","type":"family","start":447,"end":470},{"id":"IPR008373","name":"Saposin","type":"family","start":476,"end":497}],"reasoning":"I begin by dissecting the InterPro architecture and its order along the polypeptide. The N-terminus carries IPR003119 (Saposin A-type domain, residues 19–59), followed by a dense array of saposin-like modules that define a multi-repeat, lipid-interacting scaffold. Immediately downstream, IPR011001 (Saposin-like homologous superfamily) appears at residues 61–143 and is refined by IPR008139 (Saposin B type domain, residues 60–144) and IPR007856 (Saposin-like type B, region 1, residues 63–99), indicating a canonical saposin-B fold that binds and remodels membranes. This first B-type block is followed by additional B-type modules: IPR011001 (residues 182–254) and IPR008139 (residues 180–258) define a second B-type region; IPR011001 (residues 291–368) and IPR008139 (residues 290–370) mark a third; and IPR011001 (residues 393–471) plus IPR008139 (residues 392–473) complete a fourth B-type region. Between these blocks, IPR008373 (Saposin family) signatures cluster at multiple positions—19–43, 48–68, 78–95, 130–148, 294–314, 342–360, 393–415, 425–447, and 447–470—reinforcing that the entire core is a saposin-repeat scaffold. The C-terminus closes with IPR003119 (A-type domain, residues 475–515), restoring an A-type fold after the B-type array. The global family assignments IPR021165 (Saposin, chordata, residues 2–511) and IPR051428 (Pulmonary surfactant-associated protein B-like, residues 181–471) unify these repeats into a vertebrate saposin-like protein class specialized for lipid binding, membrane remodeling, and vesicular trafficking.\n\nThis ordered A–B–A–B–A–B architecture causes avid, multivalent lipid and protein interactions rather than enzymatic catalysis. Saposin folds expose amphipathic surfaces that bind membranes and lipoprotein particles; they oligomerize and remodel bilayers, which in turn creates transient protein–protein interfaces. This structural logic supports GO:0005515 protein binding as the molecular function: the repeats generate a polyvalent binding platform that recruits partners in vesicles and extracellular complexes.\n\nFrom this binding-centric mechanism, I infer anti-apoptotic and trafficking roles. Saposin-like scaffolds stabilize membranes and sequester pro-apoptotic assemblies; by buffering mitochondrial and endolysosomal membrane integrity and by dampening caspase-activating complexes, they exert GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process. The same membrane-organizing activity explains anti-apoptotic behavior and cytoprotection in diverse cell types.\n\nCellular localization follows from the fold’s membrane affinity and trafficking propensity. The absence of transmembrane helices and the presence of soluble saposin repeats indicate a luminal/vesicular and secreted distribution rather than integral membrane residence. The protein is therefore expected in the lumen of cytoplasmic vesicles and lysosome-related compartments and in the extracellular space, consistent with secretion and extracellular vesicles. A cytosolic pool (GO:0005829 cytosol) is also plausible during biosynthesis and vesicle cycling, where soluble saposin-like proteins transiently sample cytosolic pathways before vesicular import.\n\nMechanistically, I hypothesize that the multi-saposin array binds and clusters phospholipids to form membrane-curving assemblies that chaperone cargo between endosomes and lysosomes and that stabilize surfactant-associated assemblies. By organizing lipoprotein particles and vesicle membranes, the protein likely engages extracellular vesicles and secreted complexes. Partners are expected to include membrane-remodeling and trafficking factors (e.g., ESCRT-associated adaptors and tetraspanin-rich microdomains), apoptotic regulators (caspase-suppressing complexes), and surfactant-associated assemblies. These interactions collectively explain its anti-apoptotic activity and broad cytoprotective role.","category":"dark","control_evaluation":null,"validation_plan":null,"structure":{"source":"AlphaFold DB","pdb":"/structures/AF-Q6NUJ1.pdb","alphafold":"https://alphafold.ebi.ac.uk/entry/Q6NUJ1"},"links":{"uniprot":"https://www.uniprot.org/uniprotkb/Q6NUJ1/entry","atlas":"/p/Q6NUJ1"},"provenance":{"model":"BioReason-Pro (ESM3 esm3-sm-open-v1)","source_list":"Pharos Tdark","note":"AI prediction, not experimentally verified"}}