{"uniprot":"Q6ZMN8","gene":"CCNI2","name":"Cyclin-I2","organism":"Homo sapiens (Human)","status":"dark","length":369,"sequence":"MASGAQLPPQPSSSEVSAVQSPGGRPGAGLEETALGVPLPPSPGEAPLPRSNRSRCPGTRQPGAASLHAASAAVPVRPRRGTAPAGKTADAVPAAAPEQAPRPAPQSRKPRNLEGDLDERRLLCHLQLAQDREARLWRGGKPQDEICDAFEEVVLWLLRLQNTFYFSQSTFNLALTIFGRLLISVKVKEKYLHCATITSLRLAAKVNEEEEFIPQVKDFTKHYGSDYSPNELLRMELAILDRLHWDLYIGTPLDFLTIFHALVVLSWPHVLELLPQRNPSLHVASLTRQLQHCMAGHQLLQFKGSTLALVIITLELERLMPGWCAPISDLLKKAQVGDMQYSCCKELVMQQLRSLQSSSCTDNFVSPAN","function_summary":"A nuclear scaffold that uses a cyclin-box fold to bind regulatory partners and assemble nucleoplasmic complexes controlling proliferation. By organizing cyclin-dependent kinase modules and transcriptional regulators, it modulates checkpoints and gene-expression pathways that couple cell-cycle progression with broader proliferative and differentiation cues in the nucleus.","plain_language":"CCNI2 has barely been studied. The model's best guess: A nuclear scaffold that uses a cyclin-box fold to bind regulatory partners and assemble nucleoplasmic complexes controlling proliferation. By organizing cyclin-dependent kinase modules and transcriptional regulators, it modulates checkpoints and gene-expression pathways that couple cell-cycle progression with broader proliferative and differentiation cues in the nucleus.","uniprot_summary":"Involved in cell proliferation.","go_terms":{"molecular_function":[{"id":"GO:0005515","label":"protein binding"}],"biological_process":[{"id":"GO:0051726","label":"regulation of cell cycle"}],"cellular_component":[{"id":"GO:0005654","label":"nucleoplasm"}]},"interpro":[{"id":"IPR036915","name":"Cyclin-like superfamily","type":"homologous_superfamily","start":152,"end":247},{"id":"IPR006671","name":"Cyclin, N-terminal","type":"domain","start":153,"end":247},{"id":"IPR013763","name":"Cyclin-like domain","type":"domain","start":155,"end":241},{"id":"IPR039361","name":"Cyclin","type":"family","start":152,"end":348}],"reasoning":"I begin with the InterPro architecture. A compact cyclin-box core is evident: IPR006671 (Cyclin, N-terminal domain, residues 153–247) and IPR013763 (Cyclin-like domain, residues 155–241) define the canonical cyclin fold that mediates high-affinity docking to cyclin-dependent kinases (CDKs) and other regulatory partners. This core sits within IPR036915 (Cyclin-like superfamily, residues 152–247), confirming the conserved helical architecture that creates the hydrophobic patch used for partner recognition. The broader family assignment IPR039361 (Cyclin family, residues 152–348) extends beyond the minimal fold, indicating additional C-terminal elements that often tune subcellular localization and partner specificity. The ordered layout—an N-terminal cyclin box followed by extended family-specific regions—causes a regulatory scaffold that binds proteins rather than catalyzing chemistry directly, aligning with GO:0005515 protein binding as the molecular function.\n\nA cyclin-box scaffold typically governs cell-cycle transitions by assembling and modulating CDK complexes and associated transcriptional regulators. By stabilizing or redirecting CDK targeting and substrate access, this architecture regulates checkpoints and transcriptional programs that drive proliferation, which maps to GO:0051726 regulation of cell cycle. The absence of enzymatic motifs and the presence of interaction-centric cyclin domains argue that the protein acts as a context-dependent regulator rather than a kinase itself.\n\nCellular localization follows from function and sequence features. Cyclin-mediated control of transcription and checkpoint machinery is executed in the nuclear interior, and the soluble, non-membranous architecture supports a nucleoplasmic residence. This reasoning supports GO:0005654 nucleoplasm and is consistent with a nuclear subcellular location. The extended family region (152–348) likely contributes basic patches or short targeting motifs that bias the protein toward the nucleoplasm.\n\nMechanistically, the cyclin-box binds CDKs and other nuclear regulators to tune phosphorylation cascades and gene-expression modules that couple proliferation with differentiation. I hypothesize that it transiently assembles with CDK–cyclin modules and chromatin-associated factors to gate cell-cycle progression and transcriptional output. Likely partners include CDKs and canonical cyclins that share docking surfaces, as well as nuclear transport and checkpoint proteins that stabilize nucleoplasmic assemblies. Together, these interactions position the protein as a nuclear scaffold that coordinates cell-cycle regulation with broader proliferative programs.","category":"dark","control_evaluation":null,"validation_plan":null,"structure":{"source":"AlphaFold DB","pdb":"/structures/AF-Q6ZMN8.pdb","alphafold":"https://alphafold.ebi.ac.uk/entry/Q6ZMN8"},"links":{"uniprot":"https://www.uniprot.org/uniprotkb/Q6ZMN8/entry","atlas":"/p/Q6ZMN8"},"provenance":{"model":"BioReason-Pro (ESM3 esm3-sm-open-v1)","source_list":"Pharos Tdark","note":"AI prediction, not experimentally verified"}}